{"id":259,"date":"2015-05-22T15:33:20","date_gmt":"2015-05-22T19:33:20","guid":{"rendered":"https:\/\/people.clas.ufl.edu\/ebraun\/?page_id=259"},"modified":"2026-03-19T08:29:19","modified_gmt":"2026-03-19T12:29:19","slug":"nat03","status":"publish","type":"page","link":"https:\/\/people.clas.ufl.edu\/ebraun\/publications\/nat03\/","title":{"rendered":"The genome sequence of the filamentous fungus Neurospora crassa"},"content":{"rendered":"\r\n
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The genome sequence of the filamentous fungus Neurospora crassa<\/h1>\n\n\n\n

Nature<\/em>, Vol. 422, pp. 859-868<\/p>\n\n\n\n\n\n

Link to the\u00a0Nature<\/a><\/em>\u00a0home page.<\/p>\n\n\n\n\n\n

Full text is\u00a0available\u00a0from the journal for subscribers.<\/p>\n\n\n\n

The genome sequence of the filamentous fungus\u00a0Neurospora crassa<\/i><\/h3>\n\n\n\n

James E. Galagan*<\/sup>,<\/wbr>\u00a0Sarah E. Calvo,<\/wbr>\u00a0Katherine A. Borkovich,<\/wbr>\u00a0<\/sup>Eric U. Selker,<\/wbr>\u00a0Nick D. Read,<\/wbr>\u00a0<\/sup>David Jaffe,<\/wbr>\u00a0William FitzHugh,<\/wbr>\u00a0<\/sup>Li-Jun Ma,<\/wbr>\u00a0Serge Smirnov,<\/wbr>\u00a0<\/sup>Seth Purcell,<\/wbr>\u00a0Bushra Rehman,<\/wbr>\u00a0<\/sup>Timothy Elkins,<\/wbr>\u00a0Reinhard Engels,<\/wbr>\u00a0Shunguang Wang,<\/wbr>\u00a0<\/sup>Cydney B. Nielsen,<\/wbr>\u00a0Jonathan Butler,<\/wbr>\u00a0<\/sup>Matthew Endrizzi,<\/wbr>\u00a0Dayong Qui,<\/wbr>\u00a0<\/sup>Peter Ianakiev,<\/wbr>\u00a0Deborah Bell-Pedersen,<\/wbr>\u00a0<\/sup>Mary Anne Nelson,<\/wbr>\u00a0Margaret Werner-Washburne,<\/wbr>\u00a0<\/sup>Claude P. Selitrennikoff,<\/wbr>\u00a0John A. Kinsey,<\/wbr>\u00a0<\/sup>Edward L. Braun,<\/wbr>\u00a0Alex Zelter,<\/wbr>\u00a0<\/sup>Ulrich Schulte,<\/wbr>\u00a0Gregory O. Kothe,<\/wbr>\u00a0<\/sup>Gregory Jedd,<\/wbr>\u00a0Werner Mewes,<\/wbr>\u00a0<\/sup>Chuck Staben,<\/wbr>\u00a0Edward Marcotte,<\/wbr>\u00a0<\/sup>David Greenberg,<\/wbr>\u00a0Alice Roy,<\/wbr>\u00a0<\/sup>Karen Foley,<\/wbr>\u00a0Jerome Naylor,<\/wbr>\u00a0<\/sup>Nicole Stange-Thomann,<\/wbr>\u00a0Robert Barrett,<\/wbr>\u00a0<\/sup>Sante Gnerre,<\/wbr>\u00a0Michael Kamal,<\/wbr>\u00a0<\/sup>Manolis Kamvysselis,<\/wbr>\u00a0Evan Mauceli,<\/wbr>\u00a0Cord Bielke,<\/wbr>\u00a0<\/sup>Stephen Rudd,<\/wbr>\u00a0Dmitrij Frishman,<\/wbr>Svetlana Krystofova,<\/wbr>\u00a0Carolyn Rasmussen,<\/wbr>\u00a0Robert L. Metzenberg,<\/wbr>\u00a0<\/sup>David D. Perkins,<\/wbr>\u00a0Scott Kroken,<\/wbr>\u00a0<\/sup>Carlo Cogoni,<\/wbr>\u00a0Giuseppe Macino,<\/wbr>\u00a0<\/sup>David Catcheside,<\/wbr>\u00a0Weixi Li,<\/wbr>\u00a0<\/sup>Robert J. Pratt,<\/wbr>\u00a0Stephen A. Osmani,<\/wbr>\u00a0<\/sup>Colin P. C. DeSouza,<\/wbr>\u00a0Louise Glass,<\/wbr>\u00a0Marc J. Orbach,<\/wbr>\u00a0<\/sup>J. Andrew Berglund,<\/wbr>\u00a0Rodger Voelker,<\/wbr>\u00a0<\/sup>Oded Yarden,<\/wbr>\u00a0Michael Plamann,<\/wbr>\u00a0<\/sup>Stephan Seiler,<\/wbr>\u00a0Jay Dunlap,<\/wbr>\u00a0<\/sup>Alan Radford,<\/wbr>\u00a0Rodolfo Aramayo,<\/wbr>\u00a0<\/sup>Donald O. Natvig,<\/wbr>\u00a0Lisa A. Alex,<\/wbr>\u00a0<\/sup>Gertrud Mannhaupt,<\/wbr>\u00a0Daniel J. Ebbole,<\/wbr>\u00a0<\/sup>Michael Freitag,<\/wbr>\u00a0Ian Paulsen,<\/wbr>\u00a0<\/sup>Matthew S. Sachs,<\/wbr>\u00a0Eric S. Lander,<\/wbr>\u00a0<\/sup>Chad Nusbaum,<\/wbr>\u00a0and<\/wbr>\u00a0Bruce Birren*<\/sup><\/strong><\/p>\n\n\n\n\n\n

The full list of author affiliations is available from the journal\u00a0web site<\/a>. Sequencing of the\u00a0Neurospora crassa<\/i>\u00a0genome was conducted by the\u00a0Whitehead Institute\/MIT Center for Genome Research<\/a>\u00a0and the WICGR database for\u00a0Neurospora<\/i>\u00a0is available\u00a0here<\/a>.<\/p>\n\n\n\n\n\n

Neurospora crassa,<\/i>\u00a0is a\u00a0<\/sup>central organism in the\u00a0<\/sup>history of twentieth-century\u00a0<\/sup>genetics, biochemistry and\u00a0<\/sup>molecular biology.\u00a0<\/sup>Here, we report a\u00a0<\/sup>high-quality draft sequence\u00a0<\/sup>of the\u00a0N. crassa<\/i>\u00a0<\/sup>genome. The\u00a0<\/sup>approximately 40-megabase\u00a0<\/sup>genome encodes about\u00a0<\/sup>10,000 protein-codinggenes\u2039more than twice as\u00a0<\/sup>many as in the\u00a0<\/sup>fission yeast\u00a0Schizosaccharomyces\u00a0<\/sup>pombe<\/i>\u00a0and only about\u00a0<\/sup>25% fewer than\u00a0<\/sup>in the fruitfly\u00a0<\/sup>Drosophila melanogaster<\/i>.\u00a0<\/sup>Analysis of the gene\u00a0<\/sup>set yields insights into\u00a0<\/sup>unexpected aspects of\u00a0<\/sup>Neurospora<\/i>\u00a0biology\u00a0<\/sup>including the identification\u00a0<\/sup>of genes potentially associated\u00a0<\/sup>with red light\u00a0<\/sup>photobiology, genes implicated\u00a0<\/sup>in secondary metabolism,\u00a0<\/sup>and important differences\u00a0<\/sup>in Ca2+<\/sup>\u00a0signalling as compared\u00a0<\/sup>with plants and animals.\u00a0<\/sup>Neurospora<\/i>\u00a0<\/sup>possesses the widest array\u00a0<\/sup>of genome defence\u00a0<\/sup>mechanisms known for\u00a0<\/sup>any eukaryotic organism,\u00a0<\/sup>including a process unique\u00a0<\/sup>to fungi called\u00a0<\/sup>repeat-induced point mutation\u00a0<\/sup>(RIP).\u00a0<\/sup>Genome analysis suggests\u00a0<\/sup>that RIP has had a\u00a0<\/sup>profound impact on genome\u00a0<\/sup>evolution, greatly slowing the\u00a0<\/sup>creation of new genes\u00a0<\/sup>through genomic duplication\u00a0<\/sup>and resulting in a\u00a0<\/sup>genome with an unusually\u00a0<\/sup>low proportion of\u00a0<\/sup>closely related genes.<\/p>\n\n\n\n

This work was supported by the National Science Foundation with additional funding from the Deutsche Forschungsgemeinschaft, The Israel Science Foundation and the National Institutes of Health<\/p>\n\n\n\n\n\n

*<\/sup>\u00a0Corresponding authors<\/p>\n\n\n\r\n\t\t\t<\/div>\r\n\t\t<\/div>\r\n\t<\/div>\r\n<\/section>\r\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":544,"featured_media":0,"parent":19,"menu_order":21,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"featured_post":"","footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-259","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/people.clas.ufl.edu\/ebraun\/wp-json\/wp\/v2\/pages\/259","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/people.clas.ufl.edu\/ebraun\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/people.clas.ufl.edu\/ebraun\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/people.clas.ufl.edu\/ebraun\/wp-json\/wp\/v2\/users\/544"}],"replies":[{"embeddable":true,"href":"https:\/\/people.clas.ufl.edu\/ebraun\/wp-json\/wp\/v2\/comments?post=259"}],"version-history":[{"count":6,"href":"https:\/\/people.clas.ufl.edu\/ebraun\/wp-json\/wp\/v2\/pages\/259\/revisions"}],"predecessor-version":[{"id":460,"href":"https:\/\/people.clas.ufl.edu\/ebraun\/wp-json\/wp\/v2\/pages\/259\/revisions\/460"}],"up":[{"embeddable":true,"href":"https:\/\/people.clas.ufl.edu\/ebraun\/wp-json\/wp\/v2\/pages\/19"}],"wp:attachment":[{"href":"https:\/\/people.clas.ufl.edu\/ebraun\/wp-json\/wp\/v2\/media?parent=259"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}