{"id":330,"date":"2015-05-26T10:08:43","date_gmt":"2015-05-26T14:08:43","guid":{"rendered":"https:\/\/people.clas.ufl.edu\/ebraun\/?page_id=330"},"modified":"2026-03-19T08:29:19","modified_gmt":"2026-03-19T12:29:19","slug":"mbe01","status":"publish","type":"page","link":"https:\/\/people.clas.ufl.edu\/ebraun\/publications\/mbe01\/","title":{"rendered":"Fungal Zuotin Proteins Evolved from MIDA1-like Factors by Lineage-Specific Loss of MYB Domains"},"content":{"rendered":"\r\n
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Fungal Zuotin Proteins Evolved from MIDA1-like Factors by Lineage-Specific Loss of MYB Domains<\/h1>\n\n\n\n

Molecular Biology and Evolution<\/em>, Vol. 18, pp. 1401-1412<\/p>\n\n\n\n\n\n

Link to\u00a0Molecular Biology and Evolution<\/em>\u00a0home page.<\/p>\n\n\n\n\n\n

Full text is\u00a0available\u00a0from the journal.<\/p>\n\n\n\n

Fungal Zuotin Proteins Evolved from MIDA1-like Factors by Lineage-Specific Loss of MYB Domains<\/h3>\n\n\n\n

Edward L. Braun*<\/sup>\u00a0and<\/wbr>\u00a0Erich Grotewold<\/strong><\/p>\n\n\n\n\n\n

Department of Plant Biology and Plant Biotechnology Center, Ohio State University, Columbus, Ohio 43210\u00a0(both authors);
\nE.L.B.<\/a>\u00a0is currently in the Department of Zoology at the University of Florida.<\/p>\n\n\n\n\n\n

Proteins are often characterized\u00a0<\/sup>by the presence of multiple\u00a0<\/sup>domains, which make specific\u00a0<\/sup>contributions to their cellular\u00a0<\/sup>function. While the gain of domains\u00a0<\/sup>in proteins by duplication\u00a0and shuffling is well\u00a0<\/sup>established, domain loss is poorly documented.\u00a0<\/sup>Here, we provide evidence thatdomain loss has played an important\u00a0<\/sup>role in the evolution of protein\u00a0<\/sup>architecture and function by\u00a0<\/sup>demonstrating that fungal Zuotin\u00a0<\/sup>proteins evolved from MIDA1-like\u00a0proteins, present in animals and\u00a0<\/sup>plants, by complete loss of\u00a0<\/sup>the carboxyl-terminal MYB\u00a0<\/sup>domains. Phylogenetic analyses of\u00a0<\/sup>the DnaJ motif (the J domain) present\u00a0<\/sup>in both Zuotin and MIDA1\u00a0<\/sup>proteins were complicated by\u00a0<\/sup>the limited length and profound\u00a0<\/sup>differences in evolutionary rates\u00a0<\/sup>exhibited by this domain.\u00a0<\/sup>To rigorously examine J domain\u00a0<\/sup>phylogeny, we combined the nonparametricbootstrap with Monte Carlo simulation.\u00a0<\/sup>This method, which we\u00a0<\/sup>have designated the resampled parametric\u00a0<\/sup>bootstrap, allowed\u00a0<\/sup>us to assess type I and type II error\u00a0<\/sup>associated with these\u00a0<\/sup>analyses. These results revealed\u00a0<\/sup>significant support for domain\u00a0<\/sup>loss rather than domain gain or\u00a0<\/sup>gene loss involving paralogs.\u00a0The absence of sequences related\u00a0<\/sup>to the MIDA1 MYB domains in\u00a0<\/sup>Saccharomyces cerevisiae<\/i>\u00a0<\/sup>further indicates that the domains\u00a0<\/sup>have been completely lost,\u00a0<\/sup>consistent with known functional\u00a0<\/sup>differences between Zuotin\u00a0<\/sup>and MIDA1 proteins. These analysessuggest that the description of\u00a0<\/sup>additional examples of complete\u00a0<\/sup>domain loss may provide a\u00a0<\/sup>method to identify orthologous proteins\u00a0<\/sup>exhibiting functional differences\u00a0<\/sup>using genomic sequence data.<\/p>\n\n\n\n

This work was supported by grants from the USDA (1999-01582 to E.L.B.), the National Science Foundation (grant MCB-9896111 to E.G.), and Pioneer Hi-Bred International (to E.G.) and the Ohio State University Office of Research (to E.G.).<\/p>\n\n\n\n\n\n

*<\/sup>\u00a0Corresponding author<\/p>\n\n\n\r\n\t\t\t<\/div>\r\n\t\t<\/div>\r\n\t<\/div>\r\n<\/section>\r\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":544,"featured_media":0,"parent":19,"menu_order":26,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"featured_post":"","footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-330","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/people.clas.ufl.edu\/ebraun\/wp-json\/wp\/v2\/pages\/330","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/people.clas.ufl.edu\/ebraun\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/people.clas.ufl.edu\/ebraun\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/people.clas.ufl.edu\/ebraun\/wp-json\/wp\/v2\/users\/544"}],"replies":[{"embeddable":true,"href":"https:\/\/people.clas.ufl.edu\/ebraun\/wp-json\/wp\/v2\/comments?post=330"}],"version-history":[{"count":6,"href":"https:\/\/people.clas.ufl.edu\/ebraun\/wp-json\/wp\/v2\/pages\/330\/revisions"}],"predecessor-version":[{"id":455,"href":"https:\/\/people.clas.ufl.edu\/ebraun\/wp-json\/wp\/v2\/pages\/330\/revisions\/455"}],"up":[{"embeddable":true,"href":"https:\/\/people.clas.ufl.edu\/ebraun\/wp-json\/wp\/v2\/pages\/19"}],"wp:attachment":[{"href":"https:\/\/people.clas.ufl.edu\/ebraun\/wp-json\/wp\/v2\/media?parent=330"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}